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Multiplex Droplet Digital Polymerase Chain Reaction Assay for Rapid Molecular Detection of Pathogens in Patients With Sepsis: Protocol for an Assay Development Study

Multiplex Droplet Digital Polymerase Chain Reaction Assay for Rapid Molecular Detection of Pathogens in Patients With Sepsis: Protocol for an Assay Development Study

Zhang et al [38] demonstrated similar results, with a detection rate up to 80%-90% of Staphylococcus aureus and E coli in blood when using dd PCR; both studies had promising results. In this study, we will investigate dd PCR as a novel technique for sepsis diagnosis from whole blood and blood culture after 3 and 72 hours of incubation.

Samir Badran, Ming Chen, John E Coia

JMIR Res Protoc 2021;10(12):e33746